Table 5 Purification of antioxidant peptide from black eelpout muscle hydrolysate by pepsin treatment
| Purification step | EC50 value (μg/mL) | Purification folda |
|---|---|---|
| Pepsin hydrolysate | 830.01 ± 0.05 | 1.00 |
| Sephadex gel filtration (B) | 650.32 ± 0.14 | 1.28 |
| RP−HPLC (F1) | 87.45 ± 0.05 | 9.49 |
| Purified peptide (F1–1) | 56.12 ± 0.01 | 16.24 |