Validation of housekeeping genes as candidate internal references for quantitative expression studies in healthy and nervous necrosis virus-infected seven-band grouper (Hyporthodus septemfasciatus)

Table 1 Sequence, efficiency and correlation coefficient of the primers used in the present study

Gene	Primer sequence (5′-3′)	Efficiency E (%)	Correlation coefficient (R²)
Actin-β	AGCAGGAGTACGATGAGTCC GTATGCAGAGCCAGTCGTTTG	102	0.995
Glyceraldehyde-3-phoospate dehydrogenase (GAPDH)	TGCCCACGCAAACATCATTC TGAATGCCATGCCTGTCAGC	104	0.994
Acidic ribosomal protein (ARP)	GCCACGTGGAAGTCCAACTA ACAGACGGATGGTCTGCATC	101	0.999
Elongation factor 1-α (EF1α)	CGAGAAGTTCGAGAAGGAAGC GATGAGCTGCTTCACACCAAG	100	0.992

ISSN: 2234-1757