Table 1 Primer sets used in real time-quantitative PCR
| Primer information | |
|---|---|
| Primer | Sequence (5’-3’) |
| MCH Forward | CTGTTGTCGTCAGCTCTCCG |
| MCH Reverse | GGGATGCAGATCTACCCACTG |
| POMC Forward | GAACATCCGAGCTGTCAGGA |
| POMC Reverse | CATGGAGTAGGAGCGCTTGG |
| MC1R Forward | GTTGGCCATCTTCAAGAACCG |
| MC1R Reverse | TCACGTCGATGACGTTGTCC |
| ASIP Forward | GGCCCACCTCCTGTTGTTATT |
| ASIP Reverse | GACAGTTCTGAAGAGCCGACA |
| β-actin Forward | GAGGGGTATCCTGACCCTGA |
| β-actin Reverse | CTCCTCAGGGGCAACTCTC |